Summary
Type: SPE
Application #: 301900
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
2 column volumes methanol, then 2 column volumes dist. water, which was
adjusted to pH 8.5 with ammonia
Sample application:
Pour sample through the column with 3 – 4 ml / min
Column washing:
2 x 500 μl water, which was adjusted to pH 8.5 with ammonia; dry column under
vacuum for 3 – 4 min
Elution:
2 x 750 μl methanol
Authors: Macherey-Nagel
Source: LC Department